Introduction: Informed of the cancer diagnosis significantly impressed and reduced life satisfaction in the patients with cancer. It seems that religious and spiritual elements according to their components effects life satisfaction positively and increases it.This study aimed to determine the relationship between spiritual well-being and religious attitudes with life satisfaction in cancer patients. Methods: This cross-sectional study was performed on 100 cancer patients through the sequential convenience sampling method in the period of time from October to December in 2013. Data were collected by using four-part questionnaire demographic, Ellison and pollution Spiritual well-being, Glock and stark and life satisfaction Inventory-Z. Data were analyzed via SPSS version 16.0. Result: The mean score of life satisfaction was 16.03±5.58. The significant difference was seen between men (17.21±5.91) and women (14.52±4.77) with life satisfaction score (p=0.001). The overall mean of spiritual well-being and religious attitude were 78.48±12.11 and 91.24±6.52 respectively, that shows there were no substantial difference between spiritual well-being and religious attitude in both gender. The significant correlations between the Spiritual well-being with life satisfaction (p<0.001, r=0.422) and the religious attitude with life satisfaction (p=0.006, r=0.275) were been found. Conclusion: The results of this study indicated that the spiritual well-being and religious attitude have an importance effect on life satisfaction Therefore, It is recommended that nurses and health clinical staff increase life satisfaction by consideration to spiritual well-being and religious attitude in the cancer patients.